TABLE 1.

Lactate dehydrogenase activity in F. tularensis culture filtrate and cell lysate

F. tularensis strain	Growth time (h)a	OD540	Amt of protein (μg/ml)		LDH activity (mU/ml) (mean ± SE)d
			CFb	BLc	CF	BL
LVS	5	0.503	0.49	112	0.000 ± 0.000	10.008 ± 0.284
	14	0.718	0.40	120	0.008 ± 0.006	8.360 ± 0.455
RCI	5	0.544	0.39	100	0.004 ± 0.000	12.098 ± 0.171
	14	1.984	0.84	333	0.002 ± 0.003	37.259 ± 0.171

^aF. tularensis LVS and RCI were grown in Chamberlain medium for 5 or 14 h.

^bCF, culture filtrate.

^cBL, sonicated bacterial lysate.

^dLDH assay was performed as described in Materials and Methods. The experiment was performed twice with similar results.