TABLE 2.

N-terminal amino acid sequence of protein spots from 2-DE of F. tularensis culture filtrate proteins

Spota	N-terminal sequence	Best homologyb	Protein name or abbreviation	Calculated mass (kDa)c	Calculated pIc
1	EDTTTKNDNLSPQSVDLS	Catalase-peroxidase	KatG	80	5.4
2	GKIIGIDLGTTNS	70-kDa chaperone	DnaK	69	4.8
3	AAKQVLFSDEAR	60-kDa chaperone	GroEL	57	4.9
4	MNLHEYQAKDLL	Succinyl-CoA synthetase, β subunit	SucC	42	5.2
5	MRVVINGFG	Glyceraldehyde 3-phosphate dehydrogenase	GAPDH	35	5.7
6	SKTAVVFPGQGS	(Acyl carrier protein) S-melanoyltransferase	FabD	33	5.0
7	SVLVDKNT	Succinyl-CoA synthetase, α subunit	SucC	30	6.4
8	SLPTPFI	Purine-nucleoside phosphorylase	DeoD	27	7.8
9	MKFELPKLPYAV	Superoxide dismutase	SodB	22	5.6
10	TKKVPNVTFKTR	Peroxiredoxin	Ahp1	20	5.1
11	MELQLEN-QEIId	Bacterioferritin	Bfr	17	5.6
12	MNIRPLQ	10-kDa chaperone	GroES	10	5.4

^aThe spot numbers correspond to those indicated in Fig. 4.

^bThe protein with which the N-terminal sequence showed the best homology. Best homology is assigned on the basis of BLAST search results using the entire protein sequence. CoA, coenzyme A.

^cMass and pI were calculated from the entire protein sequence.

^dThe unidentified amino acid residue in the N-terminal sequence is indicated by a hyphen.