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. 2006 Jul;74(7):4190–4199. doi: 10.1128/IAI.00926-05

FIG. 1.

FIG. 1.

(A) Schematic map of the E. coli O26 LEE O26 locus indicating the position of the direct repeats (DR1 and DR2) and the integrase gene. Adjacent E. coli K-12 regions are indicated, as well as the PCR-amplified regions that were cloned to construct plasmids pMMG-4 and pMMG-7, respectively. (B) Schematic diagram of plasmids pMMG-4 (pGP704 derivative containing DR1) and pMMG-7 (pWSK129 derivative containing DR2 and the LEE integrase) indicating the mechanism of homologous recombination between the DRs and the resulting cointegrate structure. Shown also are the primer concentrations used to monitor site-specific cointegration.