TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| 413/89-1 | Stx-producing E. coli O26:H− wild-type, bovine isolate | 47 |
| INVαF′ | E. coli K-12 F′ endA1 recA1 hsdR17 (rK− mK−) supE44 thi-1 gyrA96 relA1 φ80 lacZΔM15 Δ(lacZYA-argF)U169 deoR λ− | Invitrogen |
| S17-λpir::RP4 | E. coli Tpr SmrrecA thi pro hsdRM+ RP4:2-Tc:Mu:Km Tn7 λ− pir | 21 |
| MG1655 | E. coli K-12 recA+ | 3 |
| MG1655recA56 srl300::Tn10 | E. coli MG1655 derivative harboring the recA56 mutant allele; P1 transduction | This study |
| MG1655Δb4271 | E. coli MG1655 derivative containing an in-frame deletion in the P4 integrase gene (b4271) located in the leuX locus | This study |
| MG1655Δb4271 recA56 srl300::Tn10 | E. coli MG1655 derivative containing an in-frame deletion in the integrase gene and recA56 allele | This study |
| E1294 | E. coli INVαF′ harboring plasmid pMMG-8; Apr | This study |
| Plasmids | ||
| pBAD/Myc-HisC | Apr | Invitrogen |
| pTSC29 | Cmr | 37 |
| pGP704 | Apr; ori R64 mob RP4 | 21 |
| pWH36 | pBluescript II SK derivative; Apr | This study |
| pWSK129 | Kmr | 46 |
| pMMG-4 | pGP704 harboring a 1.65-kb EcoRI fragment that corresponds to the initial sequence from LEE O26 containing direct repeat 1 (LEE O26-1) | This study |
| pMMG-5 | pGP704 harboring a 1.45-kb EcoRI fragment that corresponds to the final sequence from LEE O26 containing direct repeat 2 and 500 bp of LEE O26 integrase (LEE O26-2) | This study |
| pMMG-7 | pWSK129 harboring a 1.45-kb EcoRI fragment that corresponds to the final sequence from LEE O26 containing direct repeat 2 and LEE O26 integrase (LEE O26-2) | This study |
| pMMG-8 | pBAD/Myc-HisC, harboring an NcoI/BglII 1.19-kb fragment containing LEE O26 integrase gene | This study |
| pMMG-12 | pGP704 harboring an 860-bp EcoRI fragment containing only direct repeat 2 | This study |
| pMMG-15 | pGP704 harboring a EcoRI 1.19-kb fragment containing LEE O26 integrase gene | This study |
| pMMG-16 | pGP704 harboring a EcoRI 2.1-kb fragment containing LEE O26 integrase gene and direct repeat 2 | This study |
a
Cmr, chloramphenicol resistance; Smr, streptomycin resistance; Tpr, trimethoprim resistance; Apr, ampicillin resistance; Kmr, kanamycin resistance.