TABLE 2.
Oligonucleotides used in this study
| Name | Nucleotide sequencea | Amplimer |
|---|---|---|
| ML1up | TATGGACGGAGTAACAAGCC | LEE O26-1: 1.65-kb amplimer that corresponds to the first fragment of the LEE O26 locus containing direct repeat 1 |
| ML1lp | CGTCAATCGTCTGGTAGAACA | |
| ML1upEco | TTGGTTTATGGAATCTGGAGTAACAAGCCAAC | Same as above with restriction cutting sites for EcoRI and BglII, respectively |
| ML1lpBgl | CGTCAATCGAGATCTGGTAGAACATAATCTTCA | |
| ML2up | AGCAGGATTATATCTGATGAT | LEE O26-2: 1.45-kb amplimer that corresponds to the final fragment from LEE O26 containing direct repeat 2 and a 500-bp fragment of the LEE O26 integrase |
| ML2lp | ATGGTTGCTGAACGTGTGGATT | |
| ML2upBgl | AGCAGGATTAGATCTGATGATGCCAGACTGTAC | Same as above with restriction cutting sites for BglII and EcoRI, respectively |
| ML2lpEco | ATGGTTGCTGAATTCCGTGTGGATTATCCACTG | |
| Intup-Bgl | GCATTTGCCCGGAGATCTGATTACTGTTTTAATGGAGC | 1.19-kb fragment containing LEE O26 integrase; restriction cutting sites for BglII and NcoI, respectively |
| Intlp-Nco | GCGCCATGGGCCTGCTGGTACATCCTAATGG | |
| Intup-Eco | GCATTTGAATTCCCGGGATGATTACTGTTTTA | 1.19-kb fragment containing LEE O26 integrase; restriction cutting sites for EcoRI |
| Intlp-Eco | GCGCGGAATTCGCCTGCTGGTACATCCTAATGG | |
| DR2up.Eco | AGTTCAGCCGAATTCTGAATGCGTTAAAGAGGGTAC | 860-bp fragment containing repeat sequence 2; restriction cutting sites for EcoRI |
| ML2lp-Eco | ATGGTTGCTGAATTCCGTGTGGATTATCCACTG | |
| PheUup | TTGGTTTATGGACGGAGTAACAA | Used to evaluate chromosomal insertion in E. coli K-12 |
| PheUlp | GCAACGTGAAGATGTACTGGGAGA | Used to evaluate chromosomal insertion in E. coli K-12 |
| DP-ΔInt1 | CACAACGAATTCGCATACCTCTTCAGTGC | Construction of MG1655Δb4271 mutant located at locus leuX; restriction cutting sites for EcoRI and BglII, respectively |
| DP-ΔInt2 | AGATCTACAAGCAGATGCATACC | |
| DP-ΔInt3 | AGATCTGTTTATCAGTCCATTTGAATATG | |
| DP-ΔInt4 | GGTTCAGAATTCTGCTGAACAATTGCGATG |
a
Restriction sites are indicated in boldface.