TABLE 4.
Increase of recombinant events after arabinose induction for the different plasmids used in this study
| Recombination event | Avg recombination frequency (no. of doubly resistant colonies)a
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| pBAD vector onlyb
|
pGP704 derivativec
|
|||||||||||||
| pMMG-4
|
pMMG-5
|
pMMG-12
|
pMMG-15
|
pMMG-16
|
pGP704
|
|||||||||
| − | + | − | + | − | + | − | + | − | + | − | + | − | + | |
| Cointegrate formation with pMMG-7 | 8.3 | 9.7 | 30.4 | 85.7 | 39.5 | 96.0 | 64.0 | 221.2 | 7.7 | 7.9 | 87.5 | 110.5 | 8.7 | 8.3 |
| Chromosomal insertion | 10.1 | 11.8 | 216.2 | 593.0 | 224.2 | 693.7 | 237.0 | 421.5 | 11.1 | 12.3 | 112.2 | 119.5 | 9.8 | 11.0 |
a
That is, the number of doubly resistant colonies (to kanamycin and ampicillin) after transformation of 200 ng of each plasmid. Values are averages from three independent experiments.
b
That is, strain E1294 harboring pBAD vector. +, Induction with 0.2% arabinose; −, no induction with arabinose.
c
That is, strain E1294 harboring the relevant indicated plasmid. +, induction with 0.2% arabinose; −, no induction with arabinose.