TABLE 5.
Chromosomal insertion of plasmids pMMG-12 and pMMG-16 in the different mutants used in this study
| Plasmid | Strain | % Integrationa |
|---|---|---|
| pMMG-12 | Wild type | 43.3 |
| recA56 | 24.8 | |
| Δb4271 | 17.2 | |
| recA56 Δb4271 | 23.9 | |
| pMMG-16 | Wild type | 88.4 |
| recA56 | 43.3 | |
| Δb4271 | 20.7 | |
| recA56 Δb4271 | 31.3 |
a
That is, the percentage of chromosomal integration calculated with each strain using, as a reference of growth on LB medium plus ampicillin, the same strain transformed with plasmid pWH36 (Apr). Values represent an average of three independent experiments.