PCR analysis of TR genes. PCRs were performed with primer sets specific for the TR regions of LinJ16.1750, LinJ22.1590, and LinJ33.2870 and for a T. cruzi gene, using total DNAs of L. infantum (Li), L. donovani (Ld), L. major (Lm), L. amazonensis (La), and T. cruzi (Tc) as templates. Sizes are shown in base pairs.