FIG. 4.

Western blot analysis of MSP-5 knockout and MSP-4 truncated 5′ UTR lines. (A) Western blots of total material from synchronized late-blood-stage parasites comparing the 3D7 wild type with the 3D7-ΔMSP5 line across progressive WR99210 drug cycles, including lines treated with both WR99210 (WR) and ganciclovir (Gan) and three late-drug-cycle 3D7-ΔMSP5 clones probed with rabbit polyclonal anti-MSP-5 C-terminal antibodies (37). The panels below represent a loading control probed with rabbit polyclonal anti-MSP-1₁₉ antibodies (18). (B) Western blots of total material from parental 3D7 (wild type [W.T.]) and 3D7-TKMSP4/5-synchronized parasites at 32, 40, and 48 h postinvasion. Blots were probed with a rabbit polyclonal anti-MSP-4 antibody to confirm MSP-4 expression in 3D7-TKMSP4/5 and with rabbit polyclonal anti-SERA5 antibody (29) as a loading control. C, cycle; cl, clone.