FIG. 4.
The relative binding affinities of the various antibodies are similar. Synthetic β-1,2-mannotriose was used as described before (25) to obtain relative binding affinities by an ELISA inhibition assay for MAbs obtained from ori-B6.1, hp-B6.1, C3.1, and G11.2 hybridomas. Each point is the mean of three determinations, and the data shown are from a typical experiment of four independent experiments, all producing similar results. Relative binding affinities were also similar when the soluble inhibitor tested was either the intact phosphomannan complex or the acid-labile fraction containing the beta-linked oligomannosides of various lengths (data not shown). These data suggest that the mutations resulting in amino acid changes in the VH of the hp-B6.1 do not result in binding affinity changes. (A) ELISA inhibition data for the antibodies from ori-B6.1 and hp-B6.1. IC50, 50% inhibitory concentration. (B) ELISA inhibition data for the original ori-B6.1, C3.1, and G11.2 monoclonal antibodies.