FIG. 7.

MAb from the subclone of hp-B6.1 restored the ability to rapidly fix complement to the C. albicans cell surface relative to MAb from hp-B6.1. (A) The functional concentration of the test antibodies (MAbs from a subclone of hp B6.1 and hp-B6.1) was standardized, and fluorescence was quantified by flow cytometry. Note that the fluorescence was almost identical for both test antibodies. (B) The ability of antibodies from the subclone of hp-B6.1 and hp-B6.1 was assessed as described before. Similarly, C3 deposition onto yeast cells was obvious by 1 min of incubation in the presence of MAb from the subclone of hp-B6.1, whereas complement was barely detectable when MAb from hp-B6.1 was tested (B). By 4 min, both antibodies complement opsonized the yeast to approximately the same extent (D).