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. 2006 Jul;74(7):4224–4236. doi: 10.1128/IAI.01975-05

FIG. 4.

FIG. 4.

F. tularensis Schu4 figA and adjacent ORFs. (A) Schematic drawing. (B) RT-PCR analysis of possible transcriptional linkage between figA and adjacent ORFs. RT-PCR was carried out as described in Materials and Methods, with oligonucleotide primer pairs spanning intergenic regions 1 (5′-GTGCTGAAATGATTGACTATAGTCTC-3′ and 5′-CTTGTCATTGTTTATATTGTAGAAGAATA-3′), 2 (5′-AGTGCGATAAAGATGACATGC-3′ and 5′-TAGCTGCTATGATTATAAGC-3′), 3 (5′-TATCTATTTGACCTTAAATC-3′ and 5′-TGAATAATTGTTTCAATTTG-3′), and 4 (5′-TCAATACCAAGTTCTCAGAG-3′ and 5′-ACCTAAAAACCATGTTAAAAG-3′), respectively. Lanes 1, 4, 7, and 10, PCR products derived from F. tularensis LVS chromosomal DNA. The sizes of these PCR products are 547 bp (lane 1), 135 bp (lane 4), 120 bp (lane 7), and 121 bp (lane 10). Lanes 2, 5, 8, and 11, RT-PCR negative controls lacking reverse transcriptase. Lanes 3, 6, 9, and 12, products obtained when these same primers were used in an RT-PCR with F. tularensis LVS total RNA. Size markers are present on the left side of panel B.