TABLE 2.
F. tularensis LVS genes whose expression was maximally altered by growth under iron-restricted conditionsa
| F. tularensis Schu4 ORF no. | Gene identity or function | Avg fold differenceb |
|---|---|---|
| Genes up-regulated under iron-restricted growth conditions | ||
| 29c | Hypothetical protein (FigA) | 19.70 |
| 27c | Diaminopimelate decarboxylase (FigC) | 15.13 |
| 26c | Hypothetical protein (FigD) | 14.78 |
| 28c | Hypothetical protein (FigB) | 9.78 |
| 1565c | Glycosyl hydrolase, family 3, pseudogene | 4.08 |
| 1702 | Conserved hypothetical protein | 3.87 |
| 1701 | Hypothetical protein | 3.62 |
| 1707 | Conserved hypothetical protein | 3.41 |
| 1542c | Outer membrane protein | 3.29 |
| 1714c | Intracellular growth locus, subunit A (IglA) | 3.26 |
| 383 | Hypothetical protein | 3.22 |
| 989 | Hypothetical protein | 3.19 |
| 1709 | Conserved hypothetical protein | 3.09 |
| 980 | Aminotransferase, class II | 3.08 |
| 1711c | Intracellular growth locus, subunit D (IglD) | 3.06 |
| 1703 | Conserved hypothetical protein | 3.01 |
| 1700 | Conserved hypothetical protein (PdpB) | 2.99 |
| 1706 | Conserved hypothetical protein | 2.99 |
| 1717 | Major facilitator superfamily transport protein | 2.98 |
| 1712c | Intracellular growth locus, subunit C (IglC) | 2.96 |
| Genes down-regulated under iron-restricted growth conditions | ||
| 1214c | Haloacid dehalogenase-like hydrolase family protein | 0.32 |
| 881c | Amino acid permease | 0.39 |
| 265 | ABC transporter, membrane protein | 0.42 |
| 1269c | Chaperone protein, DnaK (heat shock protein family 70) | 0.43 |
| 1696 | Chaperone protein, GroEL | 0.43 |
| 1624c | Hypothetical protein | 0.44 |
| 1695 | Chaperone protein GroES | 0.44 |
| 938 | Adenosylmethionine-8-amino-7-oxononanoate aminotransferase | 0.46 |
| 331 | 30S ribosomal protein S3 | 0.47 |
| 172 | Hypothetical membrane protein, fragment | 0.48 |
| 327 | 50S ribosomal protein L23 | 0.49 |
| 413c | 1,4-α-Glucan branching enzyme | 0.49 |
| 328 | 50S ribosomal protein L2 | 0.49 |
| 140 | 50S ribosomal protein L11 | 0.50 |
| 326 | 50S ribosomal protein L4 | 0.50 |
| 1048c | Hypothetical protein | 0.51 |
| 266 | ABC transporter, ATP-binding protein | 0.51 |
| 551 | Conserved hypothetical protein, pseudogene | 0.52 |
| 999c | ZIP metal transporter family protein, pseudogene | 0.52 |
| 339 | 30S ribosomal protein S8 | 0.52 |
a
For detailed DNA microarray data, see Table S1 in the supplemental material.
b
The average fold difference indicates the gene expression level under iron-restricted conditions relative to that obtained under iron-replete conditions.