TABLE 2.

Growth of Tn mutants on various electron acceptors and arginine

ORFa	Gene name	Growth on:
		KNO3b		KNO2c		Arginined
		Anaerobic	Rescuee	Anaerobic	Rescue	Anaerobic	Rescue
PA0520	nirQ	−	+	−	+	+	+
PA0523	norC	−	+	−	+	+	+
PA0524	norB	−	+	−	+	+	+
PA0525	norD	−	+	−	+	+	+
PA1006		−	+	+	+	+	+
PA1544	anr	−	+	−	+	−	+
PA1546	hemN	−	+	−	+	−	+
PA1850		−	+	+	+	+	+
PA2637	nuoA	−	+	−	+	+	+
PA2638	nuoB	−	+	−	+	+	+
PA2639	nuoD	−	+	−	+	+	+
PA2641	nuoF	−	+	−	+	+	+
PA2642	nuoH	−	+	−	+	+	+
PA2644	nuoI	−	+	−	+	+	+
PA2645	nuoJ	−	+	−	+	+	+
PA2646	nuoK	−	+	−	+	+	+
PA2648	nuoM	−	+	−	+	+	+
PA2649	nuoN	−	+	−	+	+	+
PA3164	aroA	−	+	−	+	+	+
PA3912		−	+	−	+	−	+
PA3913		−	+	−	+	−	+
PA3918	moaC	−	+	+	+	+	+
PA4049		−	+	−	+	+	+
PA5497		−	+	−	+	−	+

^aPseudomonas genome database designation (www.pseudomonas.com).

^bAnaerobic growth of mutants on KNO₃ was determined by plating on LB plates containing 100 mM KNO₃ and incubating under anaerobic conditions for 48 h. The wild-type strain PAO1 grows on this medium after 24 h. +, growth; −, no growth.

^cAnaerobic growth of mutants on KNO₂ was determined by plating strains on LB plates containing 10 mM KNO₂ and incubating under anaerobic conditions for 72 h. Growth of the wild-type strain PAO1 appears after 72 h. +, growth; −, no growth.

^dAnaerobic growth of mutants on arginine was determined by plating on LB plates containing 40 mM l-arginine and incubating under anaerobic conditions for 5 days. Growth of the wild-type strain PAO1 appears after 5 days. +, growth; −, no growth.

^eAfter anaerobic incubation with either nitrate, nitrite, or arginine, plates were then transferred to aerobic growth conditions at 37°C for a period of 24 h to determine if growth could be restored. +, growth aerobically; −, no growth aerobically.