FIG. 1.

PCR analyses of A. pleuropneumoniae wild-type and isogenic mutant strains. Lanes: 1, A. pleuropneumoniae serotype 2 wild type; 2, A. pleuropneumoniae ΔapxIIAΔureCΔdmsA; 3, A. pleuropneumoniae ΔapxIIAΔureCΔdmsAΔhybB; 4, A. pleuropneumoniae ΔapxIIAΔureCΔdmsAΔhybBΔaspA; 5, A. pleuropneumoniae ΔapxIIAΔureCΔdmsAΔhybBΔaspAΔfur. Lanes M, size marker. The numbers to the left indicate the size of the PCR products obtained. Primers used were oDMSAdel1 and oDMSAdel2 (for the dmsA gene), o34-1f and 034-1r (for the hybB gene), oASPX and oASPY (for the aspA gene), and oFURX and oFURY (for the fur gene).