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Dot blot analysis of ocular pathogens following incubation with tear fluid. Cells were incubated with tear fluid for 90 min, centrifuged, washed, and resuspended in TBS. An aliquot was applied to nitrocellulose, and the blot was reacted with anti-gp340 (top) or the DF3 antibody to MUC1 (bottom). gp340 was associated with S. aureus (right lane), but not P. aeruginosa (left lane). The middle lane is blank. MUC1 associated with both bacterial species.
