FIG. 6.

Effects of JNK inhibition with different doses of the inhibitor SP600125 on total IL-8 release following infection. 16HBE14o− cells treated with the indicated doses of SP600125 were infected at low MOI with wild-type PA3, and total IL-8 was determined 20 h after infection and expressed as a percentage of maximal IL-8 secretion in the absence of inhibitor. Results are means of triplicate determinations; error bars are standard errors of the means. The drug exerted a significant inhibitory effect on IL-8 secretion as determined by ANOVA (P < 0.05). Background values from uninfected cells were subtracted in all cases.