FIG. 7.

AP-1 induction following infection of 16HBE14o− cells with wild-type (WT) or ΔexoU strains of PA3. Results show the changes in induction of AP-1 containing phospho-c-Jun bound specifically to its DNA recognition site after infection at high MOI (1 bacterium per 1 cell with no added antibiotics). Results are expressed relative to the value observed at time zero and are the means of triplicate determinations; error bars are standard errors of the means. Specificity of the assay for AP-1 binding was gauged by using a fivefold molar excess of free AP-1 binding site oligonucleotide; this reduced the signal to less than 5% of the total and was subtracted in the calculations shown here. Irrelevant sequence oligonucleotide produced no significant change in signal. Significant differences from wild-type values are shown with an asterisk (t test, P < 0.05).