FIG. 1.

E. coli Nissle 1917-conditioned medium increases NF-κB activity via TLR-2- and TLR-4-dependent pathways. After TLR-2 and TLR-4 transfection, E. coli Nissle 1917-conditioned medium increases NF-κB activity. HEK 293 cells were transiently transfected as described in Materials and Methods. At 24 h after transfection cells were stimulated with LP3 or LPS (concentration in ng/ml) or E. coli Nissle 1917 supernatants (EcN) (concentration in μl/ml) or controls in Dulbecco modified Eagle medium. At 20 h after stimulation, cell extracts were prepared for determination of luciferase activity by using a Luciferase Reporter Gene Assay. LP3 was used at 20 ng/ml only. Each bar represents mean ± the SEM value of three separate experiments.