FIG. 1.

Comparison of B. burgdorferi membrane-associated proteins separated with 2DE-NEPHGE and immunoblotted with mammalian sera. Membrane-associated proteins (MAP) from B. burgdorferi were separated by 2DE-NEPHGE (100 μg of MAP/gel) and immunoblotted with needle-infected rabbit serum (9, 10) (A) or tick-infected P. leucopus mouse serum (B). Acidic and basic ends are noted. Relative molecular mass markers (in kilodaltons) are indicated to the left of each gel. Proteins identified by MALDI-TOF analysis of identical silver-stained spots are annotated and listed in Table 2. Boxed areas indicate unidentified acidic proteins.