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. 2006 Jul;74(7):4083–4093. doi: 10.1128/IAI.00297-06

FIG. 1.

FIG. 1.

Expression of ZmpA and ZmpB in K56-2 zmpB and K56-2 zmpA zmpB mutants. Supernatant proteins were precipitated with 10% trichloroacetic acid, separated by Tricine-14% SDS-PAGE, and blotted to PVDF membranes. A. Coomassie blue-stained gel. B. Western blot reacted with anti-ZmpB. C. Western blot reacted with anti-ZmpA. Lanes 1, K56-2; lanes 2, K56-2 zmpB; lanes 3, K56-2 zmpB(pBS6); lanes 4, K56-2 zmpA zmpB; lanes 5, K56-2 zmpA; lanes 6, recombinant ZmpB; lanes 7, recombinant ZmpA. Solid arrow, ZmpA; open arrow, ZmpB.