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Expression and purification of ZmpB. Lane M, molecular weight markers (in thousands); lane 1, cell lysate after induction with IPTG; lane 2, uninduced crude cell extract; lane 3, purified inclusion bodies; lane 4, purified recombinant ZmpB after refolding and elution from an Ni-NTA column. Solid arrow, His-prepro-ZmpB fusion protein; open arrow, indicates the His-prepropeptide. The mature ZmpB is indicated.
