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Substrate specificity of ZmpB. Substrates were digested with ZmpB, ZmpAH465G (which has no proteolytic activity), or in the absence of enzyme (no protease) for 24 to 48 h. Proteins were separated by SDS-PAGE and stained with Coomassie blue. Collagen, fibronectin, and α2-macroglobulin were electrophoresed by SDS-7.5% PAGE. All other substrates were electrophoresed by SDS-12.5% PAGE.
