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. 2006 Jul;74(7):4083–4093. doi: 10.1128/IAI.00297-06

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Copyright © 2006, American Society for Microbiology

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FIG. 5. — Distribution of ZmpB in the B. cepacia complex. A. Southern hybridization analysis of the zmpB gene. A 0.8-kb ³²P-labeled MluI fragment encoding from Arg-35 to Asn-307 of the 323-amino-acid mature ZmpB from K56-2 was used to probe NotI-digested genomic DNA. Lane 1, K56-2; lane 2, B. cepacia LMG17997; lane 3, B. multivorans LMG13010; lane 4, B. cenocepacia J2315; lane 5, B. stabilis LMG14294; lane 6, B. stabilis C7322; lane 7, B. vietnamiensis PC259; lane 8, B. dolosa LMG18943; lane 9, B. ambifaria Cep996; lane 10, B. anthina LMG20980; lane 11, B. pyrrocinia LMG21822. B. Detection of ZmpB by Western blotting of culture supernatants. Trichloroacetic acid-precipitated culture supernatants were separated by Tricine 14%-PAGE and blotted onto PVDF membrane. Blots were reacted with anti-ZmpB. Lane 1, K56-2; lane 2, B. cepacia LMG17997; lane 3, B. multivorans LMG13010; lane 4, B. cenocepacia C6433; lane 5, B. stabilis LMG18888; lane 6, B. vietnamiensis Pc259; lane 7, B. dolosa LMG18943; lane 8, B. ambifaria CEP996; lane 9, B. anthina LMG20980; lane 10, B. pyrrocinia LMG 21822; lane 11, recombinant ZmpB.