TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description or origina | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 | Invitrogen |
| TOP10 | F−mcrA Δ(mrr-hsdRMSmcrBC) φ80lac ΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Smr) endA1 nupG | Invitrogen |
| B. cepacia | ||
| ATCC 25416 | Onion, United States | 37 |
| ATCC 17759 | Soil, Trinidad | 37 |
| Cep 509 | CF, Australia | 37 |
| LMG17997 | UTI, Sweden | 37 |
| B. multivorans | ||
| C5393 | CF, Canada | 37 |
| LMG13010 | CF, Belgium | 37 |
| C1576 | CF-e, United Kingdom | 37 |
| CF-A1-1 | CF-e, United Kingdom | 37 |
| JTC | CGD, United States | 37 |
| C1962 | Clinical, United Kingdom | 37 |
| ATCC 17616 | Soil, United States | |
| 249-2 | Laboratory, United States | 37 |
| B. cenocepacia | ||
| J2315 | CF-e, United Kingdom | 37 |
| BC7 | CF-e, Canada | 37 |
| K56-2 | CF-e, Canada | 37 |
| C5424 | CF-e, Canada | 37 |
| C6433 | CF-e, Canada | 37 |
| C1394 | CF-e, United Kingdom | 37 |
| PC184 | CF-e, United States | 37 |
| CEP511 | CF-e, Australia | 37 |
| J415 | CF, United Kingdom | 37 |
| ATCC 17765 | UTI, United Kingdom | 37 |
| PC715j | CF, Canada | 37 |
| K562-9 | K56-2 zmpA::tp; Tpr | 12 |
| K56-2 zmpB | K56-2 zmpB::tp; Tpr | This study |
| K56-2 zmpA zmpB | K56-2 zmpA::Tpr and zmpB::tc; Tpr, Tcr | This study |
| K56-dI2 | ΔcepI derivative of K56-2 | 40 |
| K56-R2 | cepR::Tn5-OT182 derivative of K56-2 | 32 |
| K56-2 cciI | cciI::Tp derivative of K56-2 | 1 |
| K56-2 cciR | cciR::Tp derivative of K56-2 | 40 |
| K56-2 cciIR | ΔPcciIR::Tp derivative of K56-2 | 40 |
| K56-2 cepR cciIR | cepR::Tp ΔPcciIR derivative of K56-2 | 40 |
| B. stabilis | ||
| LMG 14294 | CF, Belgium | 37 |
| C7322 | CF, Canada | 37 |
| LMG 14086 | Respirator, United Kingdom | 37 |
| LMG 18888 | Clinical, Belgium | 37 |
| B. vietnamiensis | ||
| PC 259 | CF, United States | 37 |
| LMG 16232 | CF, Sweden | 37 |
| FC 441 | CGD, Canada | 37 |
| LMG 10929T | Rice, Vietnam | 37 |
| B. dolosa | ||
| LO6 | CF | 9 |
| LMG 18943 | CF, United States | 9 |
| LMG 19468t | 9 | |
| B. ambifaria | ||
| ATCC 53266 | Corn roots, biocontrol, United States | 9 |
| CEP0996 | CF, Australia | 9 |
| B. anthina | ||
| W92T | Soil, United States | 9 |
| C1765 | CF, United Kingdom | 9 |
| J2552 | Rhizosphere, United Kingdom | 9 |
| LMG 20982 | D. P. Speert | |
| B. pyrrocinia | ||
| ATCC 15958T | Soil, Japan | 9 |
| ATCC 39277 | Soil, United States | 9 |
| Plasmids | ||
| pCR2.1-TOPO | TOPO-TA cloning vector; Apr Kmr | Invitrogen Life Technologies |
| pEX18Tc | Suicide vector; sacB Tcr | 22 |
| pUCP26 | Broad-host-range vector; Tcr | 60 |
| pRK2013 | ColE1 tra (RK2)+; Kmr | 18 |
| p34E-Tp | Source of trimethoprim cassette | 16 |
| p34S-Tc | Source of tetracycline cassette | 15 |
| pUCP21D6 | pUCP26 with 5.9-kb insert containing zmpB; Tcr | This study |
| pMS402 | lux-based promoter reporter plasmid; Kmr Tpr | 17 |
| pBS1 | pCR2.1-TOPO with 2.9-kb PCR fragment containing the zmpB gene; Apr Kmr | This study |
| pBS2 | pBS1 with 1.4-kb SalI deletion in zmpB and insertion of Tpr; zmpBΔ::Tpr | This study |
| pBS3 | pBS1 with 1.4-kb SalI deletion in zmpB and insertion of Tcr; zmpBΔ::Tcr | This study |
| pBS6 | pUCP26 with 2.9-kb XbaI-HindIII fragment from pBS1 containing zmpB; Tcr | This study |
| pBS8 | zmpB::luxCDABE transcriptional fusion in pMS402; Kmr Tpr | This study |
| pBS9 | pBS8 with tetracycline cassette from p34S-Tc; Kmr Tpr Tcr | This study |
| pPROEX-HTa | E. coli expression vector with His6 tag, TEV protease cleavage site, and inducible trc promoter; Apr | Invitrogen |
| pSCM4 | pPROEX-Hta with 1.7-kb fragment containing zmpB; Apr | This study |
a
UTI, urinary tract infection; CF-e, epidemic strain from CF patients; CGD, chronic granulomatous disease.