TABLE 3.
Virulence of K56-2 zmpA and zmpB mutants in a chronic respiratory infection model
| Strain | Inoculum (CFU) | Virulencea at day p.i.:
|
|||
|---|---|---|---|---|---|
| 7
|
14
|
||||
| Log CFU ml−1 | Inflammation (%) | Log CFU ml−1 | Inflammation (%) | ||
| K56-2 | 4.4 × 104 | 6.8 ± 7.0 | 49.7 ± 6.0 | 5.7 ± 5.4 | 66.8 ± 6.3 |
| K56-2 zmpA | 9.8 × 104 | ND | ND | 2.5 ± 2.7 | 10.3 ± 1.7* |
| K56-2 zmpB | 3.2 × 104 | 4.5 ± 4.5 | 22.8 ± 3.4* | 5.0 ± 5.0 | 16.2 ± 1.9* |
| K56-2 zmpAB | 6.2 × 104 | 0.9 ± 0.9 | 12.0 ± 1.8* | 2.1 ± 2.4 | 10.6 ± 1.1* |
a
Values are means ± standard deviations for four or five rats per group. *, significantly different from value for K56-2 at the same day p.i. (P < 0.05 by analysis of variance). ND, not determined.