FIG. 2.

Degradation of LL-37 by cell-free culture supernatant from overnight cultures of B. subtilis 168 (A) and B. anthracis UM23-Cl2 (B). LL-37 (5 mg/ml) was incubated in 100 μl of culture supernatant (▪), culture supernatant pretreated with 5 mM EDTA (⧫), or culture supernatant pretreated by heating for 1 h at 70°C (▴). Samples were evaluated by Western blotting with anti-LL-37 antibody, and the percentage of LL-37 present compared with the initial amount of LL-37 present at time zero was determined over time.