FIG. 3.

Intracellular half-life of TFV-DP in primary human hepatocytes. Primary human hepatocytes were incubated with 10 μM tenofovir (A) or 10 μM adefovir (B) for 24 h to accumulate diphosphate. Cells were then washed, fed fresh medium without drug, and then lysed at the indicated times for quantification of intracellular diphosphate levels by LC/MS/MS. Using a single exponential decay model, intracellular diphosphate half-lives of 95 ± 6 and 75 ± 1 h were calculated for tenofovir and adefovir, respectively.