TABLE 2.

DNA primers used for PCR amplification of DNA gyrase and topoisomerase IV of S. pneumoniae

Primer	Sequencea
gyrA
Forward	5′-GGCATGGATCCATGCAGGATAAAAATTTAG-3′
Reverse	5′-GTAATATCAGGATCCCTGCTAGGATATTTGTC-3′
gyrB
Forward	5′-GAAAAAGGGGATCCATGACAGAAGAAATCAAAAATCTGC-3′
Reverse	5′-CTTGAAGAATTCGTAACGATTATATCATATTTTTTAGACC-3′
parC
Forward	5′-GCTTTGGGATCCATGTCTAACATTCAAAACATGTCCCTGG-3′
Reverse	5′-CTTCGGATCCCTTATTTATCTTCAGTAACTACTTCC-3′,
parE
Forward	5′-GAATAGGGGATCCCTTGTGTCAAAAAAGGAAATC-3′
Reverse	5′-CACATAGGGATCCACATCCGACTCTAATTTCCAG-3′

^aRestriction sites are underlined.