TABLE 1.

Activities of alkoxyalkyl esters of CDV, cCDV, and HPMPA against VV and CPV in HEL fibroblast monolayers

Compound	VV		CPV		Cytotoxicity (μg/ml)		SIe
	EC50 (μg/ml)a	Fold decreaseb	EC50 (μg/ml)a	Fold decreaseb	MCC (μg/ml)c	CC50 (μg/ml)d	VV	CPV
HDP-CDV	0.013 ± 0.006	360	0.035 ± 0.004	390	>5	0.37 ± 0.09	28	11
ODE-CDV	0.0051 ± 0.0043	910	0.014 ± 0.001	900	>5	0.16 ± 0.11	31	11
OLP-CDV	0.019 ± 0.012	260	0.032 ± 0.000	420	>5	1.04 ± 0.51	55	33
ODBG-CDV	0.044 ± 0.017	130	0.069 ± 0.004	230	>5	0.30 ± 0.09	7	4
OLE-CDV	0.0083 ± 0.0029	570	0.018 ± 0.004	720	>5	0.18 ± 0.09	22	10
CDV	2.52 ± 1.45		6.83 ± 0.34		>50	37.9 ± 0.7	15	6
OLE-cCDV	0.0035 ± 0.0034	2,800	0.011 ± 0.005	1,500	>5	0.26 ± 0.18	74	24
cCDV	5.25 ± 1.23		8.97 ± 1.46		>50	42.4 ± 12.2	8	5
HDP-HPMPA	0.00051 ± 0.00044	670	0.0017 ± 0.0022	250	>2	0.044 ± 0.003	86	26
ODE-HPMPA	0.000023 ± 0.000023	15,300	0.000062 ± 0.000035	7,200	>2	0.01 ± 0.00	435	161
HPMPA	0.19 ± 0.10		0.24 ± 0.13		>20	2.80 ± 0.07	15	12

^aConcentration required to inhibit 50% of virus-induced CPE. The EC₅₀ values of each compound represent the mean ± standard deviation of the EC₅₀ values of at least two independent experiments.

^bFold decrease in EC₅₀ relative to that of the parental compound based on molar values, given the following molecular weights: HDP-CDV, 583.68; ODE-CDV, 581.71; OLP-CDV, 609.71; ODBG-CDV, 717.85; OLE-CDV, 595.68; CDV, 315.12; OLE-cCDV, 559.65; cCDV, 297.2; HDP-HPMPA, 585.72; ODE-HPMPA, 599.38; HPMPA, 325.

^cMinimum cytotoxic concentration required to alter cell morphology.

^dConcentration required to reduce cell growth by 50%. The CC₅₀ values of each compound represent the mean ± standard deviation of the CC₅₀ values of at least two independent experiments.

^eSelectivity index (ratio of CC₅₀ to EC₅₀).