TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference(s) |
|---|---|---|
| Strains | ||
| pabaA1 | TRB-sensitive A. nidulans strain | 15 |
| TerbA7 | TRB-resistant mutant of pabaA1 isolated after UV mutagenesis | 15 |
| TerbA10 | TRB-resistant mutant of pabaA1 isolated after UV mutagenesis | 15 |
| ATCC 13073 | TRB-sensitive A. fumigatus strain | ATCC (Manassas, VA) |
| TRBR-Ec-F389L | TRB-resistant mutant of ATCC 13073 transformed with pRG47 | This study |
| TRBS | TRB-sensitive strain ATCC 13073 transformed with empty plasmid pRG3-AMA1-HYG | This study |
| TRBR-F389L | TRB-resistant mutant of ATCC 13073 transformed with pRG48 | This study |
| TRBS | Strain derived from TRBR-F389L after plasmid eviction | This study |
| TRBS | TRB-sensitive strain ATCC 13073 transformed with pRG49 | This study |
| Plasmids | ||
| pRG3-AMA1 | Contains A. nidulans AMA1 gene | 1, 2 |
| pRG3-AMA1-HYG | pRG3-AMA1 containing 4-kb KpnI fragment of E. coli HYG resistance gene | This study |
| pRG46 | 2.5-kb fragment of ergA in pCR-TOPO-TA | This study |
| pRG47 | pRG46 containing point mutation F389L in ergA | This study |
| pRG48 | pRG3-AMA1-HYG containing 2.5-kb SmaI fragment of ergA from pRG47 | This study |
| pRG49 | pRG3-AMA1-HYG containing 2.5-kb SmaI fragment of ergA from pRG46 | This study |