Inhibition of ART on NF-κB activation induced by CpG ODN, LPS, and heat-killed E. coli. RAW264.7 cells (106/ml) were left untreated (medium only [lane A]), incubated with 40 μg of ART/ml for 2 h (ART [lane B]), or pretreated with the indicated concentration of ART and then incubated with 10 μg of CpG ODN 1826/ml (lane C), 0.1 μg of LPS/ml (lane E), or 3.5 × 107 CFU/ml of heat-killed E. coli (lane G) or else treated with only CpG ODN 1826 (lane D), 0.1 μg of LPS/ml (lane F), or 3.5 × 107 CFU of heat-killed E. coli/ml (lane H) for another 4 h. The cells were collected, and nuclear protein extracts were examined for NF-κB p65 activation by EMSA.