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. Author manuscript; available in PMC: 2006 Jul 7.
Published in final edited form as: Virology. 2005 Oct 17;344(2):468–479. doi: 10.1016/j.virol.2005.08.033

Fig. 2.

Fig. 2

Effect of gp41 point mutations on fas-mediated apoptosis in transiently transfected Jurkat cells. The mutations shown in Fig. 1 were placed in the vector pSRHS containing the cDNA for HIV-1 HXB2 gp160. Jurkat cells were transiently transfected then treated with 500 ng/ml anti-fas (CH11) for 3 h. Apoptosis was quantified by TUNEL staining and manually counting >300 cells/sample. The graph represents the mean percentage of apoptotic cells, ± SEM, of 3–6 independent experiments. Cells transfected with an Env-expressing plasmid were significantly more fas-sensitive than empty vector-transfected cells (P < 0.002), and all mutant-transfected cells had significantly reduced fas sensitivity compared to Env-transfected cells (P < 0.05).