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. 2003 Jan;47(1):188–195. doi: 10.1128/AAC.47.1.188-195.2003

FIG. 4.

FIG. 4.

Analysis of katG point mutants by native gel electrophoresis. (a) Peroxidase activity gel of katG mutant plasmids in the ΔkatG strain ATCC 35747 (lanes 1 to 7). Lane 8 shows vector control (VC) in the katG+ M. bovis (BCG) strain ATCC 35735. (b) Nondenaturing gel transferred to PVDF membrane and probed with anti-KatG polyclonal antibody. Lane assignments are identical to those in panel a. Although barely visible, the pD381G mutant does show a protein band with altered mobility (similar to the pT275P mutant).