TABLE 1.
Enzymatic activity and the MIC of INH for trans-dominant KatG mutantsa
| Plasmid | Mean peroxidase activity ± SD (103 U/mg) | Mean catalase activity ± SD (U/mg) | INH MIC (μg/ml) |
|---|---|---|---|
| VC | 13.0 ± 0.9 | 9.7 ± 0.5 | 0.5 |
| pWT | 49.8 ± 4.7 | 24.6 ± 0.5 | 0.25 |
| pR104L | 12.7 ± 2.1 | 10.4 ± 0.5 | 0.5 |
| pH108Q | 11.6 ± 5.9 | NDc | 0.5 |
| pN138S | 2.5 ± 0.2b | 6.8 ± 0.6b | 0.5 |
| pT275P | 3.9 ± 0.5b | 7.1 ± 0.1b | 500 |
| pS315T | 19.3 ± 5.8 | 16.1 ± 0.4 | 10 |
| pD381G | 5.2 ± 2.2b | 8.2 ± 0.1 | 250 |
| pR463L | 50.3 ± 6.9 | 17.2 ± 2.6 | 0.25 |
a
All plasmids transformed to M. bovis (BCG) ATCC 35735. VC denotes vector control plasmid (with no katG). Peroxidase activity is based on results for three different cell extracts. Catalase activity is based on results for two different cell extracts.
b
The enzymatic activity is significantly less (P < 0.05) than that for VC.
c
ND, not determined.