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Preparative IEF. The partially purified extract from cabbage leaves after affinity chromatography was subjected to a preparative IEF using a Mini-Rotofor equipment with an ampholytes pH range between 6.7 and 8.0. One-milliliter fractions were collected and the α-fucosidase activity (●) was measured against 2′-fucosyl-lactitol. Protein was measured by the Coomassie protein assay reagent (○).
