Table I.

Purification of α-l-fucosidase from cabbage leaves

Step	Activity	Protein	Specific Activity	Recovery	Purification
	nkat	mg	nkat mg−1	%
Crude extract	45	1,896	0.023	100	1
Ammonium	45	327	0.137	100	6
Sulfate ppt SP-Sepharose	41	177	0.231	91	10
Phenyl-Sepharose	35.2	86	0.409	78	17
Con-A Sepharose	31.7	21	1.509	70	65
Isoelectric focusing	1.5	0.798	1.879	3.3	81
Sephacryl S-200 HR	0.66	0.137	4.817	1.46	209

Purification procedures are described in “Materials and Methods.” Activity was assayed against 2′-fucosyl-lactitol.