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Southern-blot hybridization. Genomic DNA (12 μg) was digested with EcoRI (EI), EcoRV (EV), or HindIII (H), separated on 0.7% (w/v) agarose gels, blotted onto a nylon membrane, and hybridized with the full-length LeCDPK1 probe. Mr markers (in kb) are indicated with arrows.
