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Time course induction of LeCDPK1 and pin2 transcripts in response to wounding. Total RNA was extracted from wounded leaflets (A) non-wounded neighbor leaflets (B) or distal leaves (C) at the times indicated in the figure. Fifteen micrograms of total RNA was loaded in each lane and hybridized with radioactive LeCDPK1, TomLoxD (LOX), and pin2 probes. Equal RNA loading was checked with the EtBr staining. Relative expression levels of LeCDPK1 are plotted on the right.
