Table I.
qRT-PCR analyses of the expression of RpoT and plastid genes
Relative expression values were determined as 
for each studied gene in the sca3-2 mutant after normalization with those of the OTC gene, and compared with those of wild-type Col-0, to which a value of 1 was given. Numbers in parentheses indicate the range of variation of 
values of the studied gene obtained in two independent experiments using two different biological replicates and triplicate reactions. The range of variation of 
values in Col-0 for the RpoTm, RpoTmp, SCA3, rpoB, accD, clpP, rps18, and rpoC1 genes, respectively, were as follows: 0.69 to 1.44, 0.66 to 1.50, 0.78 to 1.27, 0.74 to 1.33, 0.67 to 1.42, 0.63 to 1.58, 0.68 to 1.45, and 0.66 to 1.50 in 4-d-old-seedlings; 0.72 to 1.38, 0.95 to 1.04, 0.55 to 1.81, 0.63 to 1.58, 0.97 to 1.02, 0.63 to 1.58, 0.87 to 1.14, and 0.98 to 1.01 in 12-d-old seedlings; 0.78 to 1.26, 0.46 to 2.15, 0.99 to 1.00, 0.59 to 1.67, 0.30 to 3.30, 0.82 to 1.20, 0.92 to 1.08, and 0.32 to 3.09 in rosettes. The range of variation for the RpoTm, RpoTmp, and SCA3 genes in Col-0 roots was 0.40 to 2.46, 0.33 to 3.00, and 0.40 to 2.48, respectively. N.D., Not determined.
| Genes | Normalized Transcript Levels in the sca3-2 Mutant Relative to Col-0
|
|||
|---|---|---|---|---|
| 4-d-Old Seedlings | 12-d-Old Seedlings | Rosettes | Roots | |
| RpoTm | 1.97 (1.32–2.95) | 0.67 (0.49–0.92) | 1.50 (1.40–1.60) | 1.10 (0.34–3.60) |
| RpoTmp | 1.45 (0.99–2.12) | 0.74 (0.71–0.77) | 1.38 (0.68–2.80) | 1.03 (0.32–3.31) |
| SCA3 | 0.32 (0.31–0.33) | 0.95 (0.64–1.40) | 0.72 (0.66–0.80) | 2.07 (1.13–3.79) |
| rpoB | 0.19 (0.05–0.74) | 0.54 (0.31–0.95) | 0.72 (0.26–1.96) | N.D. |
| accD | 0.33 (0.23–0.48) | 1.42 (1.02–1.97) | 1.02 (0.36–2.87) | N.D. |
| clpP | 0.34 (0.15–0.77) | 2.12 (1.56–2.87) | 0.44 (0.36–0.54) | N.D. |
| rps18 | 0.57 (0.45–0.77) | 1.09 (0.97–1.23) | 0.87 (0.79–0.96) | N.D. |
| rpoC1 | 0.33 (0.22–0.50) | 0.78 (0.64–0.96) | 0.65 (0.27–1.55) | N.D. |