Figure 1.

Binding of Hic-5 and its C-terminal fragment to GR τ2 region. (A) Yeast strain SFY526 was transformed with plasmids encoding a Gal4 AD–Hic-5 fusion protein and the indicated Gal4 DBD fusion protein. Cell extracts were assayed for β-gal activity produced by the integrated β-gal reporter gene, which was controlled by Gal4 response elements. Activity is given in units (U) as the mean and SD of triplicate samples. (B) Yeast two-hybrid analyses were conducted as in A with the use of yeast transformed with plasmids encoding the indicated Gal4 fusion proteins. Hic-5N and Hic-5C, the N-terminal (amino acids 1–200) and C-terminal (amino acids 201–444) fragments of Hic-5.