Figure 11.

Western blot analysis of HA-tagged Hic-5 in subcellular fractions of aerobic and ATP-depleted Cos-1 cells. Cos-1 cells were either untransfected (lane 8) or transfected with full-length HA-tagged Hic-5 (lanes 1–7) and either grown under aerobic conditions (lanes 1–4 and 8) or subjected to ATP depletion (lanes 5–7). Proteins present in whole cell extracts (lanes 1 and 8), CK buffer–extracted supernatants (lanes 2 and 5), DNAse I–digested supernatants (lanes 3 and 6), or nuclear matrix pellets (lanes 4 and 7) were separated by SDS-PAGE and subjected to Western blot analysis to detect HA-tagged Hic-5 (arrow). Samples in lanes 1–4 were also probed on a separate blot to detect lamin B protein (see Figure 12B, lanes 1–4).