Figure 2.

Transcriptional activation domain in Hic-5 and its N-terminal fragment. (A) CV-1 cells were transiently transfected with 0.5 μg of an expression vector for the indicated Gal4 DBD fusion protein and 0.5 μg of luciferase reporter gene GK1, controlled by Gal4 response elements. Luciferase activity of the transfected cell extracts is presented as the mean and SD of three transfected cultures. (B) Yeast were transformed with plasmids encoding the indicated Gal4 DBD fusion protein, and β-gal activity was determined as in Figure 1.