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Hic-5 acts as a coactivator for a subset of nuclear receptors (NR). CV-1 cells were transiently transfected with varying amounts of the indicated nuclear receptor expression vector, 0.5 μg of a suitable reporter gene for each nuclear receptor, and, where indicated, 0.5 μg of expression vector for HA-tagged Hic-5. ●, no Hic-5; □, plus Hic-5. Reporter genes: for GR, AR, MR, and PR, MMTV-LUC; for ER, MMTV(ERE)-LUC; for TR, MMTV(TRE)-LUC.
