FIG. 5.

PS receptors are predominantly responsible for the ERY-induced phagocytosis of apoptotic neutrophils by human AM. AM were treated with ERY at a concentration of 10 μg/ml, DEX at a concentration of 10⁻⁷M or medium containing 0.1% DMSO (CTRL) for 72 h. AM were preincubated with each inhibitor at 37°C for 30 min, and then exposed to apoptotic neutrophils after washing the macrophages with medium. The inhibitors were as follows: 1, phosphatidylcholine (PC) liposomes at 0.1 mM; 2, PS liposomes at 0.1 mM; 3, MAb 217 at 100 μg/ml; 4, control IgM MAb at 100 μg/ml; 5, RGDS at 2 mM; 6, RGES at 2 mM. Each value represents the means ± standard deviations (error bars) of three determinations. Statistical significance: *, P < 0.01 (versus PC liposomes in each treatment); **, P < 0.01 (versus PC liposomes in CTRL treatment).