Figure 3.

Influence of SA on the proteome of Arabidopsis seeds after 1 d imbibition. An equal amount (150 μg) of total protein extracts was loaded in each gel. A, A silver-stained 2D gel of total proteins from wild-type Arabidopsis seeds incubated for 24 h in water. The indicated portions of the gel, a, b, and c, are reproduced in B. B, Enlarged windows (a–c) of 2D gels as shown in A for dry mature seeds (left), seeds incubated in water for 24 h (middle), and seeds incubated in 0.5 mm SA for 24 h (right). The 13 labeled protein spots (protein nos. 70, 71, 76, 159, 167, 168, 329, 365, 505, 515, 520, 521, and 523) were identified by comparison with Arabidopsis seed protein reference maps (Gallardo et al., 2001, 2002a; http://seed.proteome.free.fr; Table I) or by mass spectrometry. Protein spot quantification was carried out as described in “Materials and Methods,” from at least three gels for each seed sample.