Figure 4.

Increased protein carbonyl levels in α subunits of 12S cruciferins following 1 d imbibition of Arabidopsis seeds in the presence of 0.5 mm SA. A, A silver-stained 2D gel of total proteins from wild-type Arabidopsis seeds incubated for 24 h in water. The indicated portion of the gel showing the migration of the α subunits of 12S Arabidopsis cruciferins (Job et al., 2005) is reproduced in B. C to E, Revelation of carbonylated proteins with the anti-DNP immunoassay in same gel window as shown in B. C, Dry mature seeds. D, Seeds incubated in water for 24 h. E, Seeds incubated in the presence of 0.5 mm SA for 24 h.