Table II.
Arabidopsis proteins whose abundance was significantly different in wild-type dry mature Ler seeds compared to NahG dry mature Ler seeds
% Cov., coverage; Exp., experimental; MM, molecular mass; Th., theoretical; WT, wild-type; AGI, Arabidopsis Genome Initiative; NahG/WT, normalized spot volume in the NahG Arabidopsis dry seeds divided by the normalized spot volume in the wild-type Arabidopsis dry seeds, from three different gels and independent extractions.
| No. | Exp. MM | Exp. pI | Arabidopsis Protein Name | % Cov. | Th. MM | Th. pI | AGI No. | Ratio NahG/WTa |
|---|---|---|---|---|---|---|---|---|
| kD | kD | |||||||
| 70b | 49.43 | 7.19 | 12S seed storage protein precursor | 22% | 52.49 | 7.68 | At5g44120 | 0.28 |
| 71b | 50.44 | 7.67 | 12S seed storage protein precursor | 34% | 52.49 | 7.68 | At5g44120 | 0.37 |
| 520c | 32.81 | 5.99 | α-Cruciferin 12S (seed storage protein fragment) | 12% | 31.63 | 9.08 | At5g44120 | <0.01 |
| 119b | 30.27 | 6.10 | α-Cruciferin 12S (seed storage protein fragment) | 36% | 29.23 | 6.49 | At5g44120 | 0.44 |
| 120b | 30.40 | 6.17 | α-Cruciferin 12S (seed storage protein fragment) | 28% | 29.23 | 6.49 | At5g44120 | 0.31 |
| 437c | 27.84 | 4.97 | α-Cruciferin 12S (seed storage protein fragment) | 21% | 22.81 | 9.30 | At1g03890 | 0.11 |
| 25b | 23.32 | 6.89 | β-Cruciferin 12S (seed storage protein fragment) | 27% | 21.20 | 6.19 | At4g28520 | 0.17 |
| 439c | 25.90 | 5.88 | β-Cruciferin 12S (seed storage protein fragment) | 21% | 23.36 | 6.96 | At5g44120 | 0.28 |
| 236c | 26.94 | 5.74 | Cupin family protein α subunit | 11% | 23.52 | 5.83 | At2g28490 | 2.55 |
| 140b | 60.26 | 4.15 | Calreticulin | 15% | 48.53 | 4.46 | At1g56340 | 0.53 |
| 438c | 25.92 | 5.39 | 20S proteasome α-subunit B (PAB1; PRC3) | 8% | 25.68 | 5.39 | At1g16470 | 0.19 |
| 440c | 27.27 | 5.88 | Expressed protein | 26% | 27.28 | 6.22 | At1g05510 | 5.38 |
| 431c | 66.68 | 5.84 | LEA | 32% | 67.18 | 5.97 | At2g42560 | 0.44 |
| 435c | 55.34 | 5.64 | Lectin family protein | 19% | 51.18 | 5.50 | At2g33070 | 0.38 |
| 436c | 52.15 | 5.93 | Lectin family protein | 27% | 49.69 | 6.03 | At3g21380 | 0.29 |
| SAHc | 46.95 | 4.80 | SAH (salicylate 1-monooxygenase) | 76% | 46.94 | 5.00 | P23262 | >100 |
Data obtained from densitometric analysis of individual spots from proteins in 2D gels stained with silver nitrate.
Listed proteins correspond to previously identified proteins (Gallardo et al., 2001, 2002a; Rajjou et al., 2004; Job et al., 2005).
Listed proteins correspond to proteins identified during this work; the peptide sequences determined are available in Supplemental Table V.