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. 2006 Jul;141(3):977–987. doi: 10.1104/pp.106.076604

Figure 1.

Figure 1.

ANT binds to a sequence within the FIL and YAB3 promoters. A, Alignment of a conserved element within the 5′ regulatory region of FIL and YAB3 to the ANT consensus site. The putative ANT binding site is underlined. Nucleotides shared between the ANT consensus binding site and the FIL and YAB3 promoters are shown in bold. A putative Kruppel binding site (−1,748 to −1,737), required for repression of FIL in the adaxial domain, is boxed. Numbers indicate positions relative to the start codons. B, Gel shift showing binding of ANT to the consensus binding site (BS15), the YAB3 promoter site, and the FIL promoter site. The YAB3 promoter fragment is a 106-bp sequence corresponding to nucleotides −1,561 to −1,456 and the FIL promoter fragment is a 131-bp sequence corresponding to nucleotides −1,763 to −1,633. Lanes 2 and 5 contain no protein. Increasing amounts of ANT protein are shown in lanes 3 and 4 (and 6 and 7). The same amount of ANT protein was used in lanes 1, 4, and 7.