Figure 3.
Size-exclusion chromatography demonstrates that SUS1 truncation mutants are all dimers. Equal volumes of total protein isolated in E. coli 100,000g supernatants of recombinants expressing full-length SUS1 or the truncation mutant listed to the left of the sections were resolved by size-exclusion chromatography and fractions probed on immunoblots (IB) with an antibody against MBP. The elution positions of size-exclusion chromatography molecular mass markers are shown above the sections, the calculated monomer Mr (MW) of each protein and its deduced oligomeric type are listed to the left of the sections.